Development of a highly inhibitor-tolerant engineered Taq DNA polymerase and qPCR system for POC MDx applications

This poster presents data demonstrating the inhibitor tolerance of StellarTaq and StellarScript HT+ using the buffers supplied with the Stellar qPCR and RT-qPCR Kits. Inhibitors include a range of blood-based samples, urine, saliva, UTM, bile salts, ethanol, GTC, humic acid and melanin. Performance was compared to that of commercially available products from other vendors.

• Data shows great inhibitor tolerance in both qPCR and RT-qPCR reactions
• A wide variety of inhibitors were tested
• The Watchmaker enzymes and buffers performed well compared to competitors