DNA Polymerases
Amplification for any Application
The Watchmaker DNA polymerase portfolio comprises a suite of enzymes designed to meet the challenges of modern molecular workflows — from routine PCR and isothermal applications to high-fidelity library amplification for NGS.
DNA Polymerases to meet your application needs
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| Equinox® Master Mix | Equinox Uracil-Tolerant Master Mix | StellarTaq™ DNAP | Taq DNAP | phi29 DNAP | Bsu DNAP, Large Fragment | T4 DNAP | DNAP I | |
| 3'→5' Exonuclease Activity (proofreading) | ✓ | ✓ | ✓ | ✓ | ✓ | |||
|---|---|---|---|---|---|---|---|---|
| Fidelity | ++++ | ++++ | + | + | +++ | + | +++ | ++ |
| 5'→3' Exonuclease Activity | ✓ | ✓ | ✓ | |||||
| Strand Displacement | ✓ | ✓ | ✓ | ✓ | ||||
| Processivity | +++ | +++ | +++ | ++ | +++ | ++ | ++ | ++ |
| Recommended for Nick Translation | ✓ | |||||||
| Extend RNA Primer | ✓ | ✓ | ✓ | ✓ | ||||
| Extension from Nick | ✓ | ✓ | ✓ | ✓ | ✓ | |||
| Resulting Ends | Blunt | Blunt | 3' A | 3' A | Blunt | 3' A | Blunt | Blunt |
| Heat Inactivation | 65°C for 10 min | 75°C for 20 min | 75°C for 10 min | 75°C for 20 min | ||||
| Optimal Extension Temperature | 72°C | 72°C | 72°C | 68°C | 30°C | 37°C | 37°C | 37°C |
| Applications | Ultra-high fidelity, low bias, NGS library amplification | Ultra-high fidelity, low bias, NGS library amplification | Fast, inhibitor tolerant, high specificity PCR/qPCR | General purpose PCR/qPCR, A-tailing | RCA, WGA, isothermal amplification | Isothermal amplification, especially low temperature | Blunting | Second strand synthesis, nick translation |
StellarTaq for extreme inhibitor tolerance
Inhibitors — ubiquitous in biological samples — can interfere with amplification, leading to inaccurate results and false negatives. Using an inhibitor-tolerant polymerase ensures reliable amplification even in the presence of challenging substances found in clinically relevant samples, essential for accurate pathogen detection. StellarTaq™ delivers leading inhibitor-tolerance for accurate and reproducible results every time.
Figure 1. StellarTaq DNA Polymerase (DNAP) shows robust ... MORE
StellarTaq for extreme inhibitor tolerance
Figure 1. StellarTaq DNA Polymerase (DNAP) shows robust inhibitor tolerance.StellarTaq DNAP, wild-type Taq DNAP, and other commercially available engineered Taq DNAPs (Suppliers T and R) were evaluated in probe-based qPCR. A 131 bp region of the SARS-CoV-2 ORF1ab gene was amplified from 20,000 copies of target DNA in the presence of increasing urine concentrations. ΔCq values were calculated: Cq (with inhibitor) - Cq (without inhibitor) and are displayed as a heat map where a lower ΔCq indicates increased DNA yield and inhibitor tolerance. StellarTaq had the highest inhibitor tolerance enabling accurate qPCR results on crude sample types.
RPA for consistent isothermal amplification over a wide dynamic range
Due to the variable nature of field-collected samples, the ability to detect targets across a broad dynamic range is essential. Watchmaker RPA enzymes deliver rapid and consistent amplification across a wide concentration range, with sensitivity ≤100 copies.
Figure 2. Consistent isothermal amplification ... MORE
RPA for consistent isothermal amplification over a wide dynamic range
Figure 2. Consistent isothermal amplification across a broad template concentration range. RPA reactions containing a range of copy numbers of a 168 bp target were run for 30 min. A consistently strong end signal was observed for all reactions between 100 and 1,000,000 copies.
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