DNA Polymerases
Amplification for any Application
The Watchmaker DNA polymerase portfolio comprises a suite of enzymes designed to meet the challenges of modern molecular workflows — from routine PCR and isothermal applications to high-fidelity library amplification for NGS.
DNA Polymerases to meet your application needs
Not seeing the full table? Rotate your phone to view in landscape mode.
| Equinox® Master Mix | Equinox Uracil-Tolerant Master Mix | StellarTaq™ DNAP | Taq DNAP | phi29 DNAP | Bsu DNAP, Large Fragment | T4 DNAP | DNAP I | |
| 3'→5' Exonuclease Activity (proofreading) | ✓ | ✓ | ✓ | ✓ | ✓ | |||
|---|---|---|---|---|---|---|---|---|
| Fidelity | ++++ | ++++ | + | + | +++ | + | +++ | ++ |
| 5'→3' Exonuclease Activity | ✓ | ✓ | ✓ | |||||
| Strand Displacement | ✓ | ✓ | ✓ | ✓ | ||||
| Processivity | +++ | +++ | +++ | ++ | +++ | ++ | ++ | ++ |
| Recommended for Nick Translation | ✓ | |||||||
| Extend RNA Primer | ✓ | ✓ | ✓ | ✓ | ||||
| Extension from Nick | ✓ | ✓ | ✓ | ✓ | ✓ | |||
| Resulting Ends | Blunt | Blunt | 3' A | 3' A | Blunt | 3' A | Blunt | Blunt |
| Heat Inactivation | 65°C for 10 min | 75°C for 20 min | 75°C for 10 min | 75°C for 20 min | ||||
| Optimal Extension Temperature | 72°C | 72°C | 72°C | 68°C | 30°C | 37°C | 37°C | 37°C |
| Applications | Ultra-high fidelity, low bias, NGS library amplification | Ultra-high fidelity, low bias, NGS library amplification | Fast, inhibitor tolerant, high specificity PCR/qPCR | General purpose PCR/qPCR, A-tailing | RCA, WGA, isothermal amplification | Isothermal amplification, especially low temperature | Blunting | Second strand synthesis, nick translation |
Extreme inhibitor tolerance for qPCR and RT-qPCR
Inhibitors common in clinical and biological samples can disrupt reverse transcription and DNA amplification, increasing the risk of reduced sensitivity or false negatives. An inhibitor-tolerant amplification system ensures reliable cDNA synthesis and target detection even from challenging sample types. StellarRT-qPCR and qPCR Kits deliver robust inhibitor tolerance for accurate, consistent detection of both DNA and RNA targets, supporting reproducible performance in real-world samples.
Figure 1. Stellar qPCR Kits demonstrate broad inhibitor tolerance ... MORE
StellarTaq for extreme inhibitor tolerance
Figure 1. Stellar qPCR Kits demonstrate broad inhibitor tolerance. Kits were tested against a range of inhibitors and compared to the performance of commercially available engineered polymerase+buffer systems or master mixes. Green indicates a Cq <34, yellow indicates a Cq between 34 – 38, and red indicates a Cq >38. Watchmaker systems consistently show higher success in amplifying the same targets at a Cq below 34 across all inhibitors compared to all other tested systems. Numbers are Cq values, with “-” indicating no amplification detected.
RPA for consistent isothermal amplification over a wide dynamic range
Due to the variable nature of field-collected samples, the ability to detect targets across a broad dynamic range is essential. Watchmaker RPA enzymes deliver rapid and consistent amplification across a wide concentration range, with sensitivity ≤100 copies.
Figure 2. Consistent isothermal amplification ... MORE
RPA for consistent isothermal amplification over a wide dynamic range
Figure 2. Consistent isothermal amplification across a broad template concentration range. RPA reactions containing a range of copy numbers of a 168 bp target were run for 30 min. A consistently strong end signal was observed for all reactions between 100 and 1,000,000 copies.