Taq DNA Polymerase is a thermostable DNA polymerase that catalyzes 5'→3' DNA synthesis. Taq DNAP has 5'→3' exonuclease activity and is deficient in 3'→5' exonuclease activity. Taq DNAP amplifies uracil-containing templates, can incorporate modified bases, and performs A-tailing on DNA products. This enzyme is provided in a non-hot start format.

One unit of Taq DNAP is the amount of enzyme that will incorporate 10 nmol of dNTP into activated calf thymus DNA in 30 minutes at 75°C.

• Probe and intercalating dye-based qPCR
• RT-qPCR
• dA tailing of DNA substrates pre-ligation
• PCR amplification of DNA fragments (≤ 5 kb)
• Homebrew NGS library prep

• 20 mM Tris-HCl, pH 7.4
• 100 mM NaCl
• 1 mM DTT
• 0.1 mM EDTA
• 50% Glycerol
• 0.5% Tween 20

Taq DNAP performs well in a range of buffers. The recommended 10X buffer composition is a standard PCR buffer. This buffer can be optimized for specific applications.

Standard 10X reaction buffer (not supplied with kit): 200 mM Tris-HCl, pH 8.3, 400 mM KCl

Please reference our technical guide for instructions for use.

On ice, combine components as specified:

* 2mM is the suggested final MgCl₂ concentration. Mg2+ concentrations can alter reaction performance and may need to be optimized. A scouting range between 1 – 6 mM MgCl₂ is recommended. Probe based applications will benefit from increased MgCl₂ concentration.

** Optimizing the enzyme concentration is recommended. A good general purpose starting point is 0.071 U/µL. Use higher concentrations of polymerase, up to 1.44 U/µL, in reactions with high concentrations of inhibitors, for fast PCR, or when no amplification is observed. Use lower concentrations of polymerase if non-specific amplification is observed.

Yes, but reverse-transcriptases are usually inhibitory to PCR enzymes and 1-step qRT-PCR assays may benefit from adding higher concentrations of polymerase.

No, Taq 5'→3' endonuclease activity targets double stranded DNA and as such will not degrade primers.

Yes they are compatible, but DNA Polymerase I is preferred.

Yes, it is generally not recommended to use Taq DNAP for primer extensions longer than 5 kb, or templates with extensive secondary structure.

Yes, this overhang can be used to ligate adapters with a T-overhang.

• Use templates shorter than 5 kb
• Optimize annealing temperature of the primers
• Use at least 0.2 μM final concentration of each primer
• Add fresh nucleotides
• For GC-rich templates, add DMSO to lower the temperature. Alternatively, Equinox Polymerase Master Mix can be used for more challenging targets

The extinction coefficient for Taq DNAP is 1.201 (mL/(mg*cm). If measuring protein concentration (A280), we recommend you use the protein’s extinction coefficient to ensure an accurate reading.

Yes! Watchmaker offers customer fills, packaging, concentrations, and labeling – including private label – designed to meet your unique needs. We offer flexible terms to serve organizations of any size, and our right-sized processes enable rapid turnaround time on customization. Please contact sales@watchmakergenomics.com to learn more about our capabilities.

Yes! Our Quality Management System has achieved ISO 13485:2016 Certification. Our certificate has been awarded for the design, development, manufacture, contract manufacture, and support of high performing reagents for genomics applications in medical research. Download the certificate here.