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StellarScript Portfolio

STELLARSCRIPT REVERSE TRANSCRIPTASE PORTFOLIO

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  • StellarScript Portfolio

Precision Enzymes to fuel cutting-edge applications

The StellarScript® Reverse Transcriptase (RT) portfolio features three M-MLV variants with reduced RNase H activity and enhanced processivity, activity, thermostability, and inhibitor tolerance in comparison to wild-type enzyme. Kits contain all required reagents for cDNA synthesis except primers. The portfolio includes:

  • StellarScript for template switching and robust performance
  • StellarScript HT for increased thermostability and inhibitor tolerance
  • StellarScript HT+ for template switching and the highest thermostability and inhibitor tolerance

Tailored reverse transcriptases to meet your application needs

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 PropertyApplication
 Optimal
Temp		Thermal
Stability		Inhibitor
Tolerance		Template
Switching		RT-qPCR/
qRT-PCR		RT-PCRRNA-seqscRNA-seq5' RACE3' RACE
StellarScript HT+42 – 65˚C+++++++++++++++++
StellarScript HT42 – 50˚C++++–+++++––+
StellarScript42˚C+++++++++

Table 1. Overview of StellarScript portfolio enzyme properties and relevant applications. "+" signs indicate strength of a property or fit of an application for a specific enzyme.

Improve specificity with increased thermostability

The high degree of secondary structure in many RNA species, such as high-GC transcripts, can make full-length generation of cDNA difficult and decrease overall assay sensitivity. Increased reverse transcription temperatures relax secondary structure, but this approach requires a highly thermostable reverse transcriptase. The StellarScript portfolio contains variants with a wide range of optimal reverse transcription temperatures to increase cDNA product specificity.

Figure 1A. Overcome RNA template secondary ... MORE

Figure 1B. Overcome RNA template secondary structure ... MORE

Improve specificity with increased thermostability

Figure 1A. Overcome RNA template secondary structure with thermostable RTs. StellarScript, StellarScript HT, and StellarScript HT+ were run in oligo-dT-primed first strand synthesis for 30 minutes using a 0.5 to 9 kb RNA ladder, as indicated. Reverse transcriptase thermostability increases from StellarScript to StellarScript HT and StellarScript HT+. 

Figure 1B. Overcome RNA template secondary structure with thermostable RTs. StellarScript, StellarScript HT, and StellarScript HT+ were run in oligo-dT-primed first strand synthesis at 42°C, 50°C, 60°C, or 65°C for 30 minutes using 10 ng total liver RNA as template. Resulting cDNA mass was assessed via qPCR. StellarScript and StellarScript HT activities drop at temperatures above 50°C, whereas StellarScript HT+ does not.

High-quality performance across a wide range of RNA inputs and qualities

The StellarScript portfolio delivers excellent performance across a wide range of RNA inputs and qualities, including highly damaged FFPE material and inputs as low as 10 pg. This provides broad application and sample type compatibility.

Figure 2A. Generate cDNA from low-input and ... MORE

Figure 2B. Generate cDNA from low-input ... MORE

High-quality performance across a wide range of RNA inputs and qualities

Figure 2A. Generate cDNA from low-input and degraded RNA samples. StellarScript, StellarScript HT, and StellarScript HT+ were run in oligo-dT and randomly primed first strand synthesis at 42°C (StellarScript) or 50°C (HT and HT+) for 40 minutes using 100 ng to 0.01 ng total liver RNA as template. Resulting cDNA yields were assessed via qPCR. All enzymes show similar sensitivity when reverse transcription is employed in their optimal temperature range.

Figure 2B. Generate cDNA from low-input and degraded RNA samples. StellarScript, StellarScript HT, and StellarScript HT+ were run in oligo-dT and randomly primed first strand synthesis at 50°C for 30 minutes using total RNA inputs from two independent FFPE blocks as indicated. Resulting cDNA yields were assessed via qPCR. All enzymes show similar sensitivity.

Discover more with Watchmaker

StellarScript® Reverse Transcriptase

Robust cDNA synthesis from as little as 10 pg with template switching activity...

READ MORE

StellarScript® HT Reverse Transcriptase

Improved thermostability and inhibitor tolerance improves compatibility with challenging applications...

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StellarScript® HT+ Reverse Transcriptase

Excellent thermostability and inhibitor tolerance, paired with template switching capability, delivers high-quality performance with a variety of samples, targets, and applications...

READ MORE

For assay developers – make realizing your next product easier

  • Navigate the complexities of productization with an experienced team who’s as committed to your success as you are
  • All aspects of our customization process are designed to serve you with speed, agility, and above all else, a commitment to quality
  • Tailored fill volumes, labeling (including white and private label), and packaging designed to your specifications
  • All products are manufactured within an ISO 13485:2016-certified QMS (download our certificate)