Precision that never slips
Polymerase slippage across highly repetitive regions makes accurate library amplification challenging, driving indel errors that can obscure true biological signal.
Engineered to address polymerase slippage in homopolymer and short tandem repeat regions, Equinox® Prime Library Amplification Kits reduce indel variance and lower overall error rates, enabling confident variant detection across applications from microsatellite instability (MSI) and minimal residual disease (MRD) testing to whole genome and exome sequencing.
Key Features and Benefits
- Reduced polymerase slippage across homopolymer regions improves indel accuracy for MSI profiling, whole genome, and exome sequencing
- High fidelity amplification reduces PCR errors to ensure sensitive variant calling
- Highly uniform GC coverage improves sequencing economy and ensures challenging genomic regions can be interrogated
- Efficient amplification enables users to minimize the number of PCR cycles to achieve desired yields
- Compatible with paramagnetic bead-based hybridization capture workflows for both somatic and germline testing applications
Applications
- Somatic oncology
- Minimal Residual Disease (MRD) assay development
- Microsatellite Instability (MSI) testing
- Homologous Recombination Deficiency (HRD) testing
- Tumor profiling, including FFPE
- Tumor mutation burden (TMB) analysis
- Liquid biopsy (cfDNA/ctDNA) studies
- Human whole genome sequencing (WGS)
- Targeted sequencing protocols employing hybridization capture
- Low-frequency variant detection
- Early detection and high-sensitivity screening assays
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Key Performance Data
Equinox Prime reduces polymerase slippage for more accurate indel calling
Homopolymer regions and other highly repetitive loci are among the most challenging sequences to accurately amplify, and slippage-induced indels are a persistent source of artifacts that can confound downstream variant analysis. Equinox Prime leverages a polymerase engineered to maintain high fidelity through these difficult repeat regions, making it well-suited for sensitive applications such as MSI profiling.
Figure 1. Equinox Prime minimizes slippage-associated artifacts ... MORE
Equinox Prime reduces polymerase slippage for more accurate indel calling
Figure 1. Equinox Prime minimizes slippage-associated artifacts. Slippage, a common source of false indel calls in sequencing data, was evaluated across homopolymer As and Ts in sequencing libraries amplified for 23 PCR cycles using Equinox Prime, KAPA EvoAmp™ ReadyMix, or KAPA HiFi HotStart. "Fraction Perfect," the proportion of UMI families in which all members contain the correct homopolymer repeat, reflects the percent of molecules with no indels. Equinox Prime has the least slippage across the range of repeat lengths, ensuring minimal sequencing artifacts.
Maximize library yields to minimize PCR cycling
Highly efficient library amplification with Equinox Prime generates robust yields at standard cycle numbers. Higher per-cycle efficiency means target yields are reached without requiring additional cycling, minimizing the accumulation of PCR-associated errors and bias in sensitive applications.
Figure 2. Equinox Prime delivers high library yields ... MORE
Maximize library yields to minimize PCR cycling
Figure 2. Equinox Prime delivers high library yields to minimize cycling and associated errors. Approximately 100 fg of human whole genome libraries were amplified in triplicate for 23 cycles following manufacturer's recommended cycling parameters. Libraries were quantified via D5000 TapeStation.
Uniform coverage across diverse GC content improves sequencing economy
Amplification bias can skew sequence coverage, leading to gaps or over-representation that reduce data quality and increase sequencing costs. Equinox Prime maintains consistent normalized coverage across a range of GC content, performing comparably to KAPA EvoAmp and KAPA HiFi. Combined with reduced slippage and higher library yield, Equinox Prime delivers a complete amplification solution without trade-offs.
Figure 3. Highly uniform sequence coverage ... MORE
Uniform coverage across diverse GC content improves sequencing economy
Figure 3. Highly uniform sequence coverage. Approximately 0.5 ng of human whole genome libraries were amplified in duplicate for 12 cycles following manufacturer's recommended cycling parameters.
