Date: Thursday, November 6
Time: 10 AM PT | 1 PM ET | 7 PM CET
OVERVIEW
Across many research areas, stable DNA methylation patterns can provide an orthogonal signal that powers classification, stratification, and biomarker discovery. Using whole-genome bisulfite sequencing (WGBS), prior studies have mapped condition-specific differentially methylated regions (DMRs) across cohorts, underscoring the value of methylome profiling in diverse tissues and sample types.
However, both bisulfite and enzymatic methylation sequencing convert unmodified cytosines to thymines, collapsing nucleotide diversity and, in the bisulfite case, damaging DNA. Reduced complexity complicates alignment, can raise read requirements, and limits library complexity.
This webinar will introduce TAPS+ — a positive 5mC readout approach — that directly converts 5-methylcytosines (5mC) and preserves the native A, T, C, G composition.
In this webinar, we will present:
- The chemistry and principles behind TAPS+ (direct 5mC readout while maintaining four-base complexity to yield five-base data)
- A comparison of TAPS+ relative to traditional WGBS and enzymatic methylation sequencing
- The application of TAPS+ for tumor profiling, including DMR identification, copy-number signal, and exploratory SNV/indel calls
Validate your login