gp32 Highlights
T4 Gene 32 (gp32) is a single-stranded DNA binding protein (SSBP) that is involved in T4 bacteriophage replication and repair. gp32 binds and stabilizes single-stranded DNA and is utilized as an additive in molecular biology assays to increase DNA yield.
- Increase efficiency and primer specificity during PCR and isothermal amplification
- Enhance yield during RT-PCR
- Custom formats, including high concentration, support lyophilization applications
- Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price point
- Highly stringent enzyme manufacturing ensures quality performance across lots
Applications
- RT-PCR
- Recombinase polymerase amplification (RPA)
- Whole genome amplification (WGA)
- Helicase-dependent amplification
- Isothermal amplification
- Restriction enzyme digestion
- Electron microscopy
Figure 1. T4 Gene 32 Protein bound to single stranded DNA during helicase-dependent polymerization.
QC Specifications
| Description | Specification |
|---|---|
| Protein Purity Assay | ≥ 99% |
| Exonuclease Assay* | Functional |
| DNA contamination Assay ( E. coli , mammalian, library)* | < 10 copies |
| Phosphatase Contamination Assay* | < 1% released |
| Endonuclease Contamination Assay* | Not detectable |
*As assessed using 25 mg of enzyme input per assay.
Properties
Storage buffer: 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 50% Glycerol
Heat inactivation: 65°C for 20 min
Molecular weight: 33.5 kDa
BROCHURES
Precision Enzymes and Proteins Brochure
Custom Genomic Solutions Enzymes MDx DNA Polymerase I Equinox Library Amplification Equinox Uracil-Tolerant Library Amplification phi29 DNAP RNase Inhibitor StellarScript RT StellarScript HT RT StellarScript HT+ RT StellarTaq DNA Polymerase T4 DNA Ligase T4 DNAP T4 Polynucleotide Kinase T4 Gene 32 Protein Taq DNAPWatchmaker Genomics offers a portfolio of precision-engineered enzymes and proteins, designed to meet the demands of high-stringency applications. We provide tailored services such as custom fills, formats, packaging, and labeling to accommodate unique specifications.
PROTOCOLS
T4 Gene 32 Protein Technical Guide
Enzymes T4 Gene 32 ProteinProtocol for T4 Gene 32 Protein (gp32) is a single-stranded DNA-binding protein from T4 bacteriophage that stabilizes ssDNA, enhancing yields in molecular biology assays like PCR and RT-PCR.
| CONFIGURATION | |
|---|---|
| Kit contents | T4 Gene 32 Protein (10 mg/mL) |
| Unit definition | 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 50% Glycerol |
| QUALITY CONTROL | |
|---|---|
| Purity (SDS-PAGE) | ≥ 99% |
| dsDNA exonuclease | <1% released |
| ssDNA exonuclease | <1% released |
| DNA contamination Assay (E. coli, mammalian, library)* | <10 copies |
| Phosphatase Contamination Assay* | <1% released |
| Endonuclease Contamination Assay* | Not detectable |
*As assessed using 25 mg of enzyme input per assay.
| SHIPPING AND HANDLING | |
|---|---|
| Shipping conditions | Ice packs |
| Storage | -20°C ± 5°C |
| Shelf life (off-the-shelf products) | ≥ 12 months |
| Shelf life (custom products) | ≥ 18 months |
What is T4 Gene 32 Protein (T4 gp32)?
T4 gp32 is a specific single strand DNA binding protein that is involved in T4 bacteriophage replication and repair. T4 gp32 binds and stabilizes single stranded DNA and is utilized as an additive in molecular biology assays to increase DNA yield.
What are the applications for T4 gp32?
- PCR
- Electron microscopy
- Restriction enzyme digests
- RT-PCR
- Whole genome amplification (WGA)
- Helicase-dependent amplification
What is the storage buffer for T4 gp32?
T4 gp32 storage buffer is 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 50% Glycerol.
How do we assess concentration of T4 gp32?
T4 gp32 is supplied at a concentration of 10 mg/mL. Concentration is assessed by nanodrop (DeNovix), blanked using the storage buffer and an extinction coefficient of E1% 11.76 g/100 mL.
How do we assess the functionality of T4 gp32?
The ssDNA binding affinity of T4 gp32 (reported as Kd) is determined by incubating a dilution series of T4 gp32 with a constant amount of fluorescently labeled ssDNA. The resulting signal change in fluorescence polarization is used to determine the binding affinity (Kd).
What is the extinction coefficient for T4 gp32?
The extinction coefficient of E1% is 11.76 g/100mL.
What are the recommended reaction setup conditions?
Please reference our technical guide for instructions for use.
Do you offer custom formats?
Yes! Watchmaker offers customer fills, packaging, concentrations, and labeling – including private label – designed to meet your unique needs. We offer flexible terms to serve organizations of any size, and our right-sized processes enable rapid turnaround time on customization. Please contact sales@watchmakergenomics.com to learn more about our capabilities.
Are you ISO 13485-certified?
Yes! Our Quality Management System has achieved ISO 13485:2016 Certification. Our certificate has been awarded for the design, development, manufacture, contract manufacture, and support of high performing reagents for genomics applications in medical research. Download the certificate here.
Not seeing the full table? Rotate your phone to view in landscape mode.
| Description | 500 µg | 5,000 µg | |
|---|---|---|---|
| T4 Gene 32 Protein (10 mg/mL) | 7K0071-50UL | 7K0071-500UL | Request a quote |
Please contact sales@watchmakergenomics.com to inquire about custom kit configurations.
Validate your login