gp32 Highlights
T4 Gene 32 (gp32) is a single-stranded DNA binding protein (SSBP) that is involved in T4 bacteriophage replication and repair. gp32 binds and stabilizes single-stranded DNA and is utilized as an additive in molecular biology assays to increase DNA yield.
gp32 is available in glycerol-free and glycerol-containing formats.
- Increase efficiency and primer specificity during PCR and isothermal amplification
- Enhance yield during RT-PCR
- Custom formats, including high concentration, support lyophilization applications
- Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price point
- Highly stringent enzyme manufacturing ensures quality performance across lots
Applications
- RT-PCR
- Recombinase Polymerase Amplification (RPA)
- Whole genome amplification (WGA)
- Helicase-dependent amplification
- Isothermal amplification
- Restriction enzyme digestion
- Electron microscopy
Figure 1. T4 Gene 32 Protein bound to single stranded DNA during helicase-dependent polymerization.
QC Specifications
| Description | Specification |
|---|---|
| Protein Purity Assay | ≥ 98% |
| Exonuclease Assay* | Functional |
| DNA contamination Assay ( E. coli , mammalian, library)* | < 10 copies |
| Phosphatase Contamination Assay* | < 1% released |
| Endonuclease Contamination Assay* | Not detectable |
| Activity Assay | Passed |
*As assessed using 25 mg of protein input per assay.
Properties
Storage buffer: 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 50% Glycerol
Heat inactivation: 65°C for 20 min
Molecular weight: 33.5 kDa
BROCHURES
Precision Enzymes and Proteins Brochure
Custom Genomic Solutions Enzymes MDx DNA Polymerase I Equinox Library Amplification Equinox Uracil-Tolerant Library Amplification phi29 DNAP RNase Inhibitor RNase H StellarScript RT StellarScript HT RT StellarScript HT+ RT StellarTaq DNA Polymerase T4 DNA Ligase T4 DNAP T4 Polynucleotide Kinase T4 Gene 32 Protein Taq DNAPWatchmaker Genomics offers a portfolio of precision-engineered enzymes and proteins, designed to meet the demands of high-stringency applications. We provide tailored services such as custom fills, formats, packaging, and labeling to accommodate unique specifications.
Recombinase Polymerase Amplification Brochure
Custom Genomic Solutions Isothermal Amplification Pathogen Detection Enzymes MDx Recombinase Polymerase Amplification (RPA) RNase H Bsu DNA Polymerase, Large Fragment T4 Gene 32 Protein T4 UvsX DNA Recombinase T4 UvsY ProteinThis brochure presents the Watchmaker Recombinase Polymerase Amplification (RPA) enzymes including T4 UvsX DNA Recombinase, T4 UvsY Protein, T4 Gene 32 Protein, and Bsu DNA Polymerase, Large Fragment.
Custom Genomic Solutions Brochure
Custom Genomic Solutions Isothermal Amplification Pathogen Detection PCR/qPCR Rolling Circle Amplification RT-PCR/RT-qPCR Enzymes MDx Equinox Library Amplification Equinox Uracil-Tolerant Library Amplification phi29 DNAP Recombinase Polymerase Amplification (RPA) RNase Inhibitor RNase H StellarScript RT StellarScript HT RT StellarScript HT+ RT StellarTaq DNA Polymerase Bsu DNA Polymerase, Large Fragment T4 DNA Ligase T4 DNAP T4 Polynucleotide Kinase T4 Gene 32 Protein Taq DNAP T4 UvsX DNA Recombinase T4 UvsY ProteinWhether you need small-scale modifications or a fully custom solution, Watchmaker’s flexible model ensures you get exactly what you need — with speed, simplicity, and scientific precision.
VIDEOS
Recombinase Polymerase Amplification (RPA) - Isothermal Pathogen Detection for Field-Based Assays
Isothermal Amplification Pathogen Detection DNA RNA Blood Microbial Plasma/cfDNA Saliva/buccal Urine Enzymes MDx Recombinase Polymerase Amplification (RPA) T4 Gene 32 ProteinLearn about the RPA and why superior speed and inhibitor tolerance mean it's replacing LAMP for field and point-of-care testing. Recorded at the 2025 Association for Molecular Pathology (AMP) conference, this workshop is an introduction to RPA and its capabilities, including multiplexing and RT-RPA.
POSTERS
Optimization of Recombinase Polymerase Amplification (RPA) highlights the importance of enzyme consistency for reproducible results
Isothermal Amplification DNA Enzymes MDx Recombinase Polymerase Amplification (RPA) Bsu DNA Polymerase, Large Fragment T4 Gene 32 Protein T4 UvsX DNA Recombinase T4 UvsY ProteinThis poster presents data showing how Watchmaker individual enzyme QC for RPA enzymes leads to reliable, consistent amplification. Effects of damaged proteins in RPA reactions are measured, as well as the effect of multiple freeze-thaw cycles. As presented at ADLM 2025.
PROTOCOLS
T4 Gene 32 Protein Technical Guide
Enzymes MDx Recombinase Polymerase Amplification (RPA) T4 Gene 32 ProteinProtocol for T4 Gene 32 Protein (gp32) is a single-stranded DNA-binding protein from T4 bacteriophage that stabilizes ssDNA, enhancing yields in molecular biology assays like PCR and RT-PCR.
| CONFIGURATION | |
|---|---|
| Kit contents | T4 Gene 32 Protein (10 mg/mL) |
| Storage buffer | Glycerol-containing: 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 50% Glycerol Glycerol-free: 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl |
| QUALITY CONTROL | |
|---|---|
| Purity (SDS-PAGE) | ≥ 98% |
| dsDNA exonuclease | < 1% released |
| ssDNA exonuclease | < 1% released |
| DNA contamination Assay (E. coli, mammalian, library)* | < 10 copies |
| Phosphatase Contamination Assay* | < 1% released |
| Endonuclease Contamination Assay* | Not detectable |
| Activity Assay | Passed |
*As assessed using 25 mg of protein input per assay.
| SHIPPING AND HANDLING | |
|---|---|
| Shipping conditions | Glycerol-containing: ice-packs Glycerol-free: dry ice |
| Storage | Glycerol-containing: -20°C ± 5°C Glycerol-free: -80°C ± 10°C |
| Shelf life (off-the-shelf products) | ≥ 12 months |
| Shelf life (custom products) | ≥ 18 months |
What is T4 Gene 32 Protein (gp32)?
gp32 is a specific single strand DNA binding protein which is involved in T4 bacteriophage replication and repair. gp32 binds and stabilizes single stranded DNA and is utilized as an additive in molecular biology assays to increase DNA yield.
Is gp32 available in glycerol-free format?
Yes, gp32 is available in both glycerol-containing and glycerol-free format which is suitable for downstream lyophilization.
What are the relevant applications for gp32?
- PCR
- Electron microscopy
- Restriction enzyme digests
- RT-PCR
- Whole genome amplification (WGA)
- Helicase-dependent amplification
- Recombinase Polymerase Amplification (RPA)
What is the storage buffer for gp32 - Glycerol-free?
- The glycerol-free storage buffer is 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl
- The glycerol-containing storage buffer is 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 50% Glycerol
What components are required for Recombinase Polymerase Amplification (RPA)?
RPA requires a number of different components some of which are supplied by Watchmaker Genomics. gp32 can be used for Recombinase Polymerase Amplification (RPA) of DNA when combined with glycerol-free Watchmaker Genomics reagents T4 UvsX DNA Recombinase (7K0124), T4 UvsY Protein (7K0126) and Bsu DNA Polymerase, Large Fragment (7K0128). Refer to the T4 Gene 32 Protein Technical Guide for other components required for RPA which should be sourced separately.
How do I set up an initial RPA reaction with gp32?
For suggestions on how to set up an initial RPA reaction, please refer to the T4 Gene 32 Protein Technical Guide .
How do I design primers for RPA?
The following characteristics are of critical importance when designing RPA primers:
- The optimal length for RPA primers is 30–35 nt. Primers less than 30 nt are not recommended.
- Primer sequences should have between 30–70% GC content and have no single or dinucleotide base repeats.
- Primer sequences should also be devoid of complementary sequences which promote secondary structure hairpin loops and prevent self-dimerization or primer-primer interactions.
- Amplicon sequences should have between 35–60% GC content, with an optimal length of 150–450 bp.
- If possible, the primer should end with a G or C on the 3' terminus.
How is gp32 inactivated?
Incubation at 65°C for 20 minutes will inactivate gp32.
Where can I go for additional help or technical support?
If you need more detailed guidance or have questions not addressed here, please reach out to our support team at support@watchmakergenomics.com or submit a request via our Technical Support form. Please include details such as product name, lot number, version of protocol you’re using, and a brief description of your question or issue so we can assist you promptly.
Do you offer custom formats?
Yes! Watchmaker offers customer fills, packaging, concentrations, and labeling – including private label – designed to meet your unique needs. We offer flexible terms to serve organizations of any size, and our right-sized processes enable rapid turnaround time on customization. Please contact sales@watchmakergenomics.com or submit a request via our Custom Solutions form to learn more about our capabilities.
Are you ISO 13485-certified?
Yes! Our Quality Management System has achieved ISO 13485:2016 Certification. Our certificate has been awarded for the design, development, manufacture, contract manufacture, and support of high performing reagents for genomics applications in medical research. Download the certificate here.
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| Description | 250 µg | 500 µg | 5,000 µg | |
|---|---|---|---|---|
| T4 Gene 32 Protein (10 mg/mL) | 7K0071-50UL | 7K0071-500UL | Request a quote | |
| T4 Gene 32 Protein - Glycerol-free (10 mg/mL) | 7K0127-25UL | 7K0127-500UL | Request a quote |
Please contact sales@watchmakergenomics.com to inquire about custom kit configurations.
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