Highlights
This application note demonstrates automated WGS library preparation on Hamilton Microlab NGS STAR platforms using the Watchmaker DNA Library Prep Kit with Fragmentation, featuring:
- Automated workflows that produce library quality comparable to manual preparation across both NGS STAR MOA and LSA configurations
- High-throughput processing of up to 96 samples with dynamic workflow options and sequencing-ready libraries generated in as little as four hours
- Robust, reproducible performance across diverse agricultural species, delivering consistent library quality, uniform sequencing metrics, and >99% alignment for most samples
