This application note evaluates the performance, reproducibility, and scalability of the Watchmaker RNA Library Prep Kit with Polaris^® Depletion automated on the Hamilton Microlab^® NGS STAR, demonstrating that both MOA and LSA configurations deliver high-quality whole-transcriptome libraries comparable to manual prep across low- and high-input workflows.

• Delivers high concordance between automated and manual RNA-seq library prep, with consistent performance across MOA and LSA configurations for whole-transcriptome profiling
• Demonstrates robust performance from low RNA inputs, enabling sensitive detection of gene expression with maintained library complexity and quality
• Shows that Watchmaker RNA Library Prep with Polaris Depletion integrates seamlessly on the Hamilton NGS STAR, delivering reproducible rRNA depletion, streamlined workflows, and scalability for high-throughput labs