PCR amplification is a critical step in NGS library preparation, and the choice of enzyme has a direct impact on library quality, coverage uniformity, and variant detection sensitivity. Traditional amplification approaches involve trade-offs between yield, GC coverage uniformity, and fidelity that become harder to ignore as sequencing applications become more demanding.

Watchmaker offers a portfolio of library amplification solutions to eliminate these compromises, supporting high-stringency applications including rare variant detection, cfDNA analysis, single-cell analysis, and targeted sequencing.

• NEW Equinox^® Prime Library Amplification Kits deliver high-fidelity amplification across repeat-rich regions for more accurate variant detection, without compromising performance on yield, GC coverage uniformity, or PCR efficiency.
• Equinox HC (High Concentration) Library Amplification Kits are a more concentrated (4X) master mix, for library amplification reactions from dilute inputs.
• Equinox Uracil Tolerant Library Amplification Kits enable amplification of uracil-containing templates including bisulfite-converted or deaminated DNA.

Already using Equinox Library Amplification Kits? It remains a trusted choice with a proven track record across a broad range of NGS applications.

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