mRNA-seq workflows are often slow and labor-intensive, requiring multiple handling and cleanup steps that can compromise sensitivity and sequencing efficiency, especially at low RNA inputs.
The Watchmaker mRNA Library Prep Kit is engineered to generate stranded, high-complexity libraries in under 5 hours, delivering improved gene detection sensitivity and better use of sequencing reads across a broad input range.
Designed for improved sensitivity and coverage from a streamlined workflow
Download the brochure to learn how to:
- Build stranded mRNA libraries in a single workday (< 5 hours)
- Generate high-complexity libraries from inputs as low as 2.5 ng
- Simplify automation with on-magnet washing and fewer handling steps
See the data on gene detection sensitivity, reduced data distortion, and uniform transcript coverage with as little as 2.5 ng RNA.
DOWNLOAD THE BROCHURE
Download the brochure
Process more samples easily with a simple, rapid workflow
Improve automatability and reduce turnaround time. The Watchmaker mRNA Library Prep Kit combines and shortens reaction steps and has fewer cleanups than other commercially available kits. On-magnet bead washing during mRNA capture eliminates the need for pipetting to resuspend during washes, reducing magnet time and consumable use. Generous reagent overages are included to meet dead volume requirements of liquid handlers.
Sensitive performance with broad sample inputs
Increase library complexity and gene detection sensitivity ... MORE
Maximize sequencing economy ... MORE
Sensitive performance with broad sample inputs
Increase library complexity and gene detection sensitivity with low-inputs. RNA extracted from breast tissue (RIN 7) was used to prepare libraries in quadruplicate from a range of RNA mass inputs using the Watchmaker mRNA Library Prep Kit, KAPA mRNA HyperPrep Kit, and the NEBNext® Ultra™ II Directional RNA Library Prep Kit with poly(A) mRNA Enrichment. Supplier recommendations were used for each workflow. KAPA failed to produce libraries at 2.5 ng. Libraries were downsampled to 5M reads. The Watchmaker solution detects significantly more unique genes with low-input samples and an equivalent number of genes when RNA mass is not limiting (with a much-improved workflow). Unique genes were defined as those supported by greater than or equal to 5 unique raw reads.
Sensitive performance with clinically relevant samples
Waste fewer bases to rRNA contamination. RNA extracted from breast tissue (RIN 7) was used to prepare libraries in quadruplicate from a range of RNA mass inputs, as indicated, using the Watchmaker mRNA Library Prep Kit, KAPA mRNA HyperPrep Kit, and the NEBNext® Ultra™ II Directional RNA Library Prep Kit with poly(A) mRNA Enrichment. Supplier recommendations were used for each workflow. KAPA failed to produce libraries at 2.5 ng. One replicate KAPA library was omitted from analysis due to higher than anticipated residual rRNA. Libraries were downsampled to 5M reads. The Watchmaker solution results in efficient mRNA enrichment across the full RNA input range to reduce residual rRNA.
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