Maximizing RNA sequencing economy from blood samples using a combined mRNA capture and globin depletion workflow

This technical note presents an optimized workflow that combines mRNA capture with globin and rRNA depletion to maximize sequencing efficiency from blood-derived RNA.

• Maximize sequencing economy by combining mRNA capture with globin and rRNA depletion to focus reads on informative, coding transcripts.
• Enhance data quality with reduced duplication rates, increased gene detection, and uniform 5' to 3' transcript coverage across a range of RNA inputs.
• Streamline your workflow with a single-day protocol (~6.25 hours) optimized for high-yield, high-complexity RNA-seq libraries from blood samples.