Many RNA-seq workflows are time- and labor-intensive, and commercially available kits often struggle to deliver consistent performance with challenging, clinically relevant samples.
Watchmaker offers three RNA library prep solutions that are highly sensitive, scalable, and robust with low RNA inputs and tough sample types, including FFPE tissue and blood.
Designed for reliable RNA-seq from challenging sample types
Download the brochures to learn how to:
- Increase sensitivity with FFPE and low-input samples
- Generate stranded libraries for quantitative gene expression analyses
- Improve throughput and automatability with fewer cleanups and handling steps
- Achieve consistent performance from a reverse transcriptase engineered specifically for RNA sequencing
See the data on gene detection sensitivity, high library prep efficiency, and performance with low-input and diverse RNA samples.
DOWNLOAD THE BROCHURES
Download the brochures
Process more samples easily with a simple workflow, complete in under 5 hours
Truly single-day RNA library prep. The Watchmaker RNA portfolio offers streamlined workflow options to address any RNA sequencing application and associated sample type. Reduced incubation times and fewer purification and handling steps makes all three workflows highly automatable and easily completed in a single workday.
Sensitive performance with clinically relevant samples
Watchmaker RNA Library Prep Kit with Polaris® Depletion
Improve sequencing economy and gene detection sensitivity ... MORE
Watchmaker mRNA Library Prep Kit
Increase library complexity and gene detection sensitivity ... MORE
Detect more genes across a broad input range
Improve sequencing economy and gene detection sensitivity. Libraries were prepared from a whole blood sample in triplicate using the RNA inputs indicated. Data were randomly downsampled to 16M paired reads per library. Unique genes identified using featureCounts with a cutoff of 5 deduplicated raw reads.
Sensitive performance with clinically relevant samples
Increase library complexity and gene detection sensitivity with low-inputs. RNA extracted from breast tissue (RIN 7) was used to prepare libraries in quadruplicate from a range of RNA mass inputs using the Watchmaker mRNA Library Prep Kit, KAPA mRNA HyperPrep Kit, and the NEBNext® Ultra™ II Directional RNA Library Prep Kit with poly(A) mRNA Enrichment. Supplier recommendations were used for each workflow. KAPA failed to produce libraries at 2.5 ng. Libraries were downsampled to 5M reads. The Watchmaker solution detects significantly more unique genes with low-input samples and an equivalent number of genes when RNA mass is not limiting (with a much-improved workflow). Unique genes were defined as those supported by greater than or equal to 5 unique raw reads.
EXPLORE MORE DATA
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Choose the right kit for your transcriptomic analysis
| Watchmaker RNA Library Prep Kit with Polaris Depletion | Watchmaker mRNA Library Prep Kits | Watchmaker RNA Library Prep Kit | |
| RNA enrichment method | Ribosomal and globin depletion | Bead-based poly(A) capture | None |
|---|---|---|---|
| RNA input | 1 ng to 1 µg | 2.5 ng to 1 µg | 0.25 to 100 ng |
| Library Prep Time | < 4.5 hours | < 5 hours | < 3.5 hours |
| Species compatibility | Human, mouse, and rat | Any eukaryote | Any |
| Sample type compatibility | FFPE Blood Tissue biopsies Dried blood spots* Cell lines and organoids Intact and degraded | Intact (RIN > 7) Tissue biopsies Cell lines and organoids | Any |
| Types of RNA detected | Protein-coding (exonic; mRNA) Long non-coding (lncRNA) | Protein-coding (exonic mRNA) | Protein-coding (exonic; mRNA) Long non-coding (lncRNA) |
| Includes reagents for: | rRNA/globin depletion FFPE treatment RNA library prep Library amplification | mRNA Capture RNA library prep Library amplification | FFPE treatment RNA library prep Library amplification |
Need help choosing the right approach? A Watchmaker specialist can review your application, walk through input compatibility, and help you select the right workflow. Contact our team.