Protocols for the EquiPlex Normalization Kits.
Singleplex: enables the individual normalization and pooling of 96 amplified NGS libraries without prior quantification or dilution. The Cas9-based binding workflow preserves double-stranded library integrity and accepts inputs from 2.5 to 200 nM, with libraries eluted ready for direct sequencing on Illumina platforms.
Multiplex: normalizes and pools 8 to 96 amplified NGS libraries into an equimolar pool without prior quantification or dilution. The Cas9-based binding workflow preserves double-stranded library integrity and accepts inputs from 2.5 to 200 nM, with pools eluted ready for direct sequencing on Illumina sequencing platforms.
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