RNase INHIBITOR
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Protect what's precious
RNase Inhibitor exhibits high affinity, non-competitive binding of RNases A, B, and C at a 1:1 ratio, enabling high-quality cDNA synthesis from low-quality RNA samples. The absence of two cysteines present in human and porcine RNase inhibitors make this murine version highly suitable for low-DTT applications.
- Prevent RNA degradation in RT-qPCR, single cell, and single nuclei sequencing workflows at temperatures up to 55°C
- Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price-point
- Improve performance in low-DTT settings with a murine RNase inhibitor that better resists oxidation
- Custom formats, including high concentration, support lyophilization applications
- Highly stringent enzyme manufacturing ensures quality performance across lots
Applications
- Nuclei isolation
- RT-PCR and RT-qPCR
- cDNA synthesis
- Applications where maintaining RNA integrity is critical
- Single-cell RNA sequencing
- Bulk RNA-sequencing
- Cell-free cloning
- In vitro synthesis (IVT)
Properties
Unit definition: One unit of RNase inhibitor is defined as the amount of RNase Inhibitor required to inhibit activity of 0.375 ng of RNase A by ≥ 95%
Reaction conditions:
Active at temperatures ≤ 55°C
Storage buffer: 20 mM HEPES-KOH, 0.1 mM EDTA, 50 mM KCl, 8 mM DTT, 50% glycerol, pH 7.6
Heat inactivation: 70°C for 20 min
Molecular weight: 50.6 kDa
Key Performance Data
Safeguard RNA assay performance
Ribonucleases (RNases) are ubiquitous and can have significant and detrimental impacts on assay performance and sensitivity. Incorporating an RNase inhibitor to prevent RNA degradation ensures reliability and accuracy of experimental results – particularly for studies and assays focused on pathogen detection, gene expression, RNA stability, and other RNA-dependent processes.
Figure 1A. Prevent RNA degradation through the ... MORE
Figure 1B. Prevent RNA degradation at elevated ... MORE
Safeguard RNA assay performance
Figure 1A. Prevent RNA degradation through the addition of RNase inhibitor. The addition of RNase inhibitor to first strand synthesis (FSS) reactions improves cDNA yield even when no RNase A is added to the reaction – highlighting risks of RNase contamination during sample handling. Addition of 5 ng RNase A results in a >10-fold decrease in cDNA yield when no RNase inhibitor is present. Assay performance is rescued by the addition of RNase inhibitor, where all commercial enzymes evaluated perform equivalently. RNase Inhibitors sourced from a variety of vendors were added to oligo-dT-primed FSS reactions with StellarScript HT+ Reverse Transcriptase. Reactions ran for 30 min at 42°C using 10 ng total liver RNA.
Figure 1B. Prevent RNA degradation at elevated temperatures through the addition of RNase Inhibitor. The addition of RNase Inhibitor to high-temperature (55°C) first strand synthesis (FSS) reactions improves cDNA yield even when no RNase A is added to the reaction – highlighting risks of RNase contamination during sample handling. Assay performance is rescued by the addition of RNase Inhibitor. RNition of 5 ng RNase A results in a >10-fold decrease in cDNA yield when no RNase Inhibitor is present. Assay ase Inhibitor was added to oligo-dT-primed FSS reactions with StellarScript HT+ Reverse Transcriptase. Reactions ran for 30 min at 55°C using 10 ng total liver RNA.
BROCHURES
Precision Enzymes and Proteins Brochure
Custom Genomic Solutions Enzymes MDx DNA Polymerase I Equinox Library Amplification Equinox Uracil-Tolerant Library Amplification phi29 DNAP RNase Inhibitor StellarScript RT StellarScript HT RT StellarScript HT+ RT StellarTaq DNA Polymerase T4 DNA Ligase T4 DNAP T4 Polynucleotide Kinase T4 Gene 32 Protein Taq DNAPWatchmaker Genomics offers a portfolio of precision-engineered enzymes and proteins, designed to meet the demands of high-stringency applications. We provide tailored services such as custom fills, formats, packaging, and labeling to accommodate unique specifications.
RNase Inhibitor Brochure
Pathogen Detection RT-PCR/RT-qPCR Single Cell Sequencing RNA Blood FFPE Microbial Saliva/buccal Enzymes MDx RNA NGS RNase InhibitorWatchmaker Genomics' RNase Inhibitor binds RNases A, B, and C, protecting RNA integrity in applications like RT-qPCR and single-cell sequencing at temperatures up to 55°C. Its murine origin enhances performance in low-DTT conditions, and custom formats support lyophilization.
PROTOCOLS
RNase Inhibitor Technical Guide
Enzymes MDx RNA NGS RNase InhibitorProtocol for Watchmaker Genomics' RNase Inhibitor which binds RNases A, B, and C, protecting RNA integrity in applications like RT-PCR and single-cell RNA-seq at temperatures up to 55°C.
| CONFIGURATION | |
|---|---|
| Kit contents | RNase Inhibitor (40 U/µL or 800 U/µL) |
| Unit definition | One unit of RNase Inhibitor is defined as the amount of RNase Inhibitor required to inhibit activity of 0.375 ng of RNase A by ≥95% |
| Enzyme storage buffer formulation | 20 mM HEPES-KOH, 0.1 mM EDTA, 50 mM KCl, 8 mM DTT, 50% glycerol, pH 7.6 |
| QUALITY CONTROL | |
|---|---|
| Protein Purity Assay | >97% |
| dsDNA exonuclease* | <1% released |
| ssDNA exonuclease* | <1% released |
| DNA contamination* (E. coli, mammalian, library) | <10 copies |
| Phosphatase assay* | <1% released |
| Endonuclease* | Not detectable |
| Nonspecific RNase* | Not detectable |
*As assessed using 450 U of protein input per assay.
| SHIPPING AND HANDLING | |
|---|---|
| Shipping conditions | Ice packs |
| Storage | -20°C ± 5°C |
| Shelf life (off-the-shelf products) | ≥ 12 months |
| Shelf life (custom products) | ≥ 18 months |
What is RNase Inhibitor?
RNase Inhibitor exhibits high-affinity, non-competitive binding of RNases A, B, and C, enabling high-quality cDNA synthesis from low-quality RNA samples.
How is one unit of RNase Inhibitor defined?
1 unit of RNase Inhibitor is defined as the amount of RNase Inhibitor required to inhibit activity of 0.375 ng of RNase A by ≥ 95%.
What are the applications for the RNase inhibitor?
- First strand synthesis
- RT-PCR, RT-qPCR
- Nuclei Isolation
- Single-cell RNA-sequencing
- in vitro synthesis (IVT)
- cDNA synthesis
- Cell-free cloning
- RNA labeling
- Any applications where maintaining RNA integrity is critical
What is the storage buffer for RNase Inhibitor?
- 20 mM HEPES-KOH
- 0.1 mM EDTA
- 50 mM KCl
- 8 mM DTT
- 50% glycerol, pH 7.6
Which RNase types is RNase inhibitor effective against?
RNase types A, B and C.
When should RNase Inhibitor be added to a reaction?
Prior to the addition of any RNA that could be digested by RNases present in any of the components.
What is the optimal temperature for RNase Inhibitor?
RNase Inhibitor is active ≤ 55°C.
Do I need to add the RNase Inhibitor when using purified RNA?
While it is not essential, we have demonstrated a significant increase in yield when RNase Inhibitor is added to RNA-based reactions.
What origin is Watchmaker's RNase Inhibitor from?
The Watchmaker RNase Inhibitor is murine in origin.
Is DTT required for RNase inhibitor activity?
The absence of two cysteines present in human and porcine RNase inhibitors make this murine version much more suitable for low-DTT applications.
Can RNase Inhibitor be inactivated?
RNase Inhibitor can be heat-inactivated by incubating at 70°C for 20 minutes.
How much RNase inhibitor should be used?
We recommended 1 U/µL of RNase Inhibitor per assay.
What is the extinction coefficient for RNase Inhibitor?
The extinction coefficient for RNase Inhibitor is 0.8 (mL/(mg*cm). If measuring protein concentration (A280), we recommend you use the protein's extinction coefficient to ensure an accurate reading.
What are the recommended reaction setup conditions?
Please reference our technical guide for instructions for use.
Do you offer custom formats?
Yes! Watchmaker offers customer fills, packaging, concentrations, and labeling - including private label - designed to meet your unique needs. We offer flexible terms to serve organizations of any size, and our right-sized processes enable rapid turnaround time on customization. Please contact sales@watchmakergenomics.com to learn more about our capabilities.
Are you ISO 13485-certified?
Yes! Our Quality Management System has achieved ISO 13485:2016 Certification. Our certificate has been awarded for the design, development, manufacture, contract manufacture, and support of high performing reagents for genomics applications in medical research. Download the certificate here.
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| Description | 4 kU | 20 kU | 40 kU | |
|---|---|---|---|---|
| RNase Inhibitor (40 U/µL) | 7K0088-100UL | 7K0088-500UL | Request a quote | |
| RNase Inhibitor (800 U/µL) | 3K0108-50UL | Request a quote |
Please contact sales@watchmakergenomics.com to inquire about custom kit configurations.
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Custom Genomic Solutions
Custom formats, white label kitting, single-lot shipments, custom certificates of analysis, advanced change notification, and flexible terms for any size organization...
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